 |
 |
|
|
|
|
ELDON CARDS FOR BLOOD GROUPING
Eldoncards for Blood Grouping are easy to use. They require no special laboratory facilities and can be done in the office or at a client's home. There is a reliable result within 3 minutes.
Eldoncards are easy to store before using. The reagents remain stable for 2 years stored at room temperature. After use: the cards can be placed in the client's file and serve as documentation for an unlimited time.
INSTRUCTIONS FOR USE
1. Drop one full drop of tap water (50 ul [microliter])
on to reagent in each test panel.
Do not use distilled water or saline.
2. Prepare the finger or the earlobe,
pierce with a sterile microlancet.
3. Touch the blood drop from below
with the 4 teeth of the plastic comb,
so that a hemisphere of blood settles
on the end of each tooth.
4. The amount of whole blood required is 10 ul.
5. Rub quickly to and fro for 30 seconds.
Spread the blood right out to all sides of the panels.
6. Wait 1 minute. In the interim, record client's
name and other data in the space provided.
7. Tilt card for 2 minutes:
back and forth and from side to side.
8. Read and report results.
In order to be certain of the homogeneous
appearance of the negative reactions, it is
recommended to tilt the card once more
2 minutes after the reading.
TYPES OF CARDS
The ABO-D Eldoncard
Contains the reagents for determining the blood group within the ABO and Rh(D) systems.
The CDE Eldoncard
Contains the reagents for determining the blood group within the Rh(CDE) system.
The Test Card
Contains the reagents (anti-A, anti-B) for controlling the identity within the ABO system between recipient and donor blood.
The Eldomatcard
A special machine for semiautomatic blood group determinations with Eldoncards. Speeds up the procedure and secures a correct use of the Eldoncard method.
READING AND INTERPRETATION OF THE RESULTS
The 8 combinations of blood groups in the ABO-and Rhesus-systems, as they appear on the Eldoncards.
Anti-A Anti-B Anti-D Control Result O-positive O-negative A-positive A-negative B-positive B-negative AB-positive AB-negative Unreadable (Use the suspension method)
CONTROL PANEL:
This panel must not show agglutination. If it does, the test is not valid.
Note: The phenomenon may be due to auto-antibodies in certain forms of hemolytic anemia or to maternal antibodies in erthroblastosis fetalis. Also bacterial transformation of the cells may cause joint agglutination in all 4 panels.
ABO GROUP:
This group is read in the anti-A and anti-B panels.
Group Anti-A Anti-B O No Agglutination No Agglutination A Agglutination No Agglutination B No Agglutination Agglutination AB Agglutination Agglutination
In the anti-A panel the reaction of A1 blood is often remarkably strong, that of A2 and A2 B moderately strong while A3 reacts with small agglutinates on an unagglutinated background. Weak A subgroups of importance in blood transfusions give clear-cut reactions on the cards.
Rh GROUP:
Agglutination in the anti-D panel indicates that the subject is Rh-positive. No agglutination demonstrates lack of the Rh factor D, and the subject must be considered Rh-negative at least as recipient.
Doubtful reaction despite retesting with correct technique is due to the fairly uncommon D variant Du (Duffy factor). As a precautionary measure Du persons are also considered Rh-negative as recipients. As donors they are Rh positive. Rh negative donors are those persons alone who lack not only the Rh-factor D, but also C and E (and Du which in Europeans is hardly ever seen except together with C or E). This may be ascertained by the CDE Eldoncards.
COMMENTS:
The dry card may be preserved with plastic (cover, tape, or spray) and handed over or filed for reference. All sera used are tested and found negative for anti-HIV and HBsAg.
SUSPENSION METHOD
This method is used if the ordinary identity-proof test shows that it is indicated (pale panels or joint agglutination in all 4 panels, including the control panel), or if only cord blood is available:
Stabilized venous blood is centrifuged for 3 minutes (3000 r.p.m.). 2 drops of the packed red blood cells are washed 3 times with 10 ml 0.9% saline solution and suspended in 20 drops of saline (1 ml). By a standard pipette held 45 degrees inclined, a large drop of the suspension (60 ul) is allowed to fall direct on to the dry reagent in each panel.
The reagents are dissolved by quick rubbing of the comb back and forth for 30 seconds, whereupon the suspension is spread right out to all sides of the panel. The card is tilted and read as usual.
STORAGE OF ELDONCARDS
If capillary blood (from finger or earlobe) is used:Keeping qualities 3 years stored at 18 - 30°C (65 - 85°F) and 5 years stored at 4°C (40°F).
If venous blood/suspensions are used:Keeping qualities 2 years stored at 24°C (75°F).
If incorrectly stored, the cellulose film becomes yellow and wrinkled, and the control paper adheres to the reagents. Such cards should not be used.
Eldoncards are manufactured by Nordisk Gentofte A/S, Niels Steensensvej 1, DK-2820 Gentofte, Denmark.
Eldoncards can be purchased from: Cascade HealthCare Products, Inc., Diagnostic Tests, Blood Typing
Other Purchasing Sources:
Eldoncard.com
Helpful Information
Press your "back" button to return to the last page.
