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ELDON CARDS FOR BLOOD GROUPING
Eldoncards for Blood Grouping are easy to use. They require no special laboratory facilities and can be done in the office or at a client's home. There is a reliable result within 3 minutes.
Eldoncards are easy to store before using. The reagents remain stable for 2 years stored at room temperature. After use: the cards can be placed in the client's file and serve as documentation for an unlimited time.
INSTRUCTIONS FOR USE
TYPES OF CARDS
Contains the reagents for determining the blood group within the ABO and Rh(D) systems.
Contains the reagents for determining the blood group within the Rh(CDE) system.
Contains the reagents (anti-A, anti-B) for controlling the identity within the ABO system between recipient and donor blood.
A special machine for semiautomatic blood group determinations with Eldoncards. Speeds up the procedure and secures a correct use of the Eldoncard method.
READING & INTERPRETATION OF THE RESULTS
The 8 combinations of blood groups in the ABO-and Rhesus-systems, as they appear on the Eldoncards.
Anti-A Anti-B Anti-D Control
CONTROL PANEL
This panel must not show agglutination. If it does, the test is not valid.
Note: The phenomenon may be due to auto-antibodies in certain forms of hemolytic anemia or to maternal antibodies in erthroblastosis fetalis. Also bacterial transformation of the cells may cause joint agglutination in all 4 panels.
ABO GROUP
This group is read in the anti-A and anti-B panels.
Group Anti-A Anti-B O No Agglutination No Agglutination A Agglutination No Agglutination B No Agglutination Agglutination AB Agglutination Agglutination
In the anti-A panel the reaction of A1 blood is often remarkably strong, that of A2 and A2B moderately strong while A3 reacts with small agglutinates on an unagglutinated background. Weak A subgroups of importance in blood transfusions give clear-cut reactions on the cards.
Rh GROUP
Agglutination in the anti-D panel indicates that the subject is Rh-positive. No agglutination demonstrates lack of the Rh factor D, and the subject must be considered Rh-negative at least as recipient.
Doubtful reaction despite retesting with correct technique is due to the fairly uncommon D variant Du (Duffy factor). As a precautionary measure Du persons are also considered Rh-negative as recipients. As donors they are Rh positive. Rh negative donors are those persons alone who lack not only the Rh-factor D, but also C and E (and Du which in Europeans is hardly ever seen except together with C or E). This may be ascertained by the CDE Eldoncards.
COMMENTS
The dry card may be preserved with plastic (cover, tape, or spray) and handed over or filed for reference. All sera used are tested and found negative for anti-HIV and HBsAg.
SUSPENSION METHOD
This method is used if the ordinary identity-proof test shows that it is indicated (pale panels or joint agglutination in all 4 panels, including the control panel), or if only cord blood is available:
Stabilized venous blood is centrifuged for 3 minutes (3000 r.p.m.). 2 drops of the packed red blood cells are washed 3 times with 10 ml 0.9% saline solution and suspended in 20 drops of saline (1 ml). By a standard pipette held 45 degrees inclined, a large drop of the suspension (60 ul) is allowed to fall direct on to the dry reagent in each panel.
The reagents are dissolved by quick rubbing of the comb back and forth for 30 seconds, whereupon the suspension is spread right out to all sides of the panel. The card is tilted and read as usual.
STORAGE OF ELDONCARDS
If capillary blood (from finger or earlobe) is used:Keeping qualities 3 years stored at 18 to 30°C (65 to 85°F) and 5 years stored at 4°C (40°F).
If venous blood/suspensions are used:Keeping qualities 2 years stored at 24°C (75°F).
If incorrectly stored, the cellulose film becomes yellow and wrinkled, and the control paper adheres to the reagents. Such cards should not be used.
Eldoncards are manufactured by Nordisk Gentofte A/S, Niels Steensensvej 1, DK-2820 Gentofte, Denmark.
ELDON CARD PURCHASING RESOURCES
Eldoncard.com
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